Journal: Cell & Bioscience

Article Title: The cytoskeleton regulates cytoophidium dynamics in Drosophila ovaries

doi: 10.1186/s13578-026-01530-1

Figure Lengend Snippet: Kinesin does not affect cytoophidia dynamic changes. A Time-lapse imaging revealed cytoophidia morphological changes, The time marked in the upper left corner represents the time since the capture began. The CTPS (gray) signal shown is obtained using mCherry-tagged CTPS. Scale bar = 5 μm. B Khc knockdown Drosophila ovaries. CTPS (red) signal shown is obtained using mCherry-tagged CTPS. Nuclei(blue) are stained by Hoechst 33342. scale bars, 20 μm. C Quantification of cytoophidia transport through nurse cell-oocyte ring canals in control and Khc knockdown groups. We analyzed 30 ovaries in control group and 25 ovaries in Khc knockdown group. D The speed of cytoophidia movement. We analyzed 41 filaments in control group and 30 filaments in Khc knockdown group, Data are represented as mean ± SEM. ns, no significance in difference. E Length of cytoophidia in ovary nurse cell. We analyzed 43 filaments in control group and 44 filaments in Khc knockdown, Data are represented as mean ± SEM. ns, no significance in difference. F Number of cytoophidia in nurse cell. We analyzed 6 ovaries in control group and 162 filaments in total, and 6 ovaries in Khc knockdown and 184 filaments in total, Data are represented as mean ± SEM. ns, no significance. G Intensity of CTPS-mCherry fluorescence signal in cytoophidia (ns, no significance in difference). 3 ovaries in control group and 3 ovaries in Khc group are analyzed. Data are represented as mean ± SEM.

Article Snippet: Hoechst 33342 (Bio-Rad, 1351304) specifically labels nuclei due to its high affinity for binding to DNA, and Rhodamine-conjugated phalloidin (1:200; SB-YP0059-50T, share-bio) was used to label the cell boundary by binding to F-actin.

Techniques: Imaging, Knockdown, Staining, Control, Fluorescence